Our previous investigations effectively employed an interstitial all-optical needle photoacoustic sensing probe on ex vivo tissue studies. In this study, our goal is to locate aggressive PCa within an intact prostate ex vivo using our latest version translational needle PA probe. Targeting specific tissue components, we utilized wavelengths of 1220nm, 1370nm, 800nm, and 266nm. Evans blue dye was injected at the measured positions for histopathology analysis. The acquired photoacoustic signals were analyzed using PASA, including spectrum slopes and midbandfits derived at all wavelengths. With the limited number of insertions, we were able to identify cancers in 3 out of 4 prostates. This non-invasive methodology holds considerable promise for future clinical applications.
In this study, we developed a prototype interstitial all-optical needle photoacoustic sensing probe for clinical translation of prostate cancer. The performance of the PA needle probe was examined on intact human prostates ex vivo to simulate the transrectal ultrasound (US) guided transperineal prostate biopsy procedures. Analysis based on PA spectrum analysis in 8-28 MHz range of acquired RF signals at multi-wavelengths shows statistical difference between benign and cancerous regions (n=49, p<0.05). Multivariate SVM analysis using linear and midbandfit parameters can obtain an accuracy close to 90%.
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