Data on the effects of high-intensity pulsed THz radiation (peak intensity ~30 GW/cm2 , electric field strength ~3.5 MV/cm) on human skin fibroblasts have been obtained for the first time. A quantitative assessment of the number of histone H2AX phosphorylation foci in a cell as a function of irradiation time and THz pulse energy was obtained. It has been shown that the appearance of foci is not associated with either oxidative (cells retain their morphology, cytoskeleton structure, and the content of reactive oxygen species does not exceed the control values) or thermal stress. Long-term irradiation of cells did not reduce their proliferative index.
We report on the investigation of the biological effects of high-power pulsed broadband terahertz (THz) radiation on human skin fibroblasts. Cells were exposed to THz pulses with peak intensity ~30 GW/cm2 and electrical field strength ~3.5 MV/cm for 1.5 hours. Phosphorylation of H2AX (γH2AX foci) can be caused by different reasons including DNA double stand breaks, heat induction and can be related to chromosomes stability. However, the γH2AX foci seem to be a proper marker for detection of the effect of intense terahertz pulses on cells. The kinetics of phosphorylated histone H2AX foci post terahertz radiation exposure is analyzed. We found that the number of phosphorylated H2AX foci in human skin fibroblasts remained almost unchanged within 24 hours following terahertz exposure.
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