We present a novel ultrafast imaging system using Spatio-Temporal Optical Coherence Tomography (STOC-T), capable of acquiring structural images of a mouse retina at a volumetric rate of 112 Hz, aided by a calibrated fundus camera for focal plane adjustment. We extract blood pulse traces from retinal and choroidal vessels using a structural-only OCT analysis, and pulse wave-induced retinal layer displacement from differential OCT phase analysis. With both analyses, we measure hemodynamic parameters, such as the delays between arterial and venous pulsation, to provide a comprehensive suite of potential biomarkers of retinal diseases.
We present a novel ultrafast imaging system using Spatio-Temporal Optical Coherence Tomography (STOC-T), capable of acquiring structural images of a mouse retina at a volumetric rate of 112 Hz. A calibrated fundus camera and white-light illumination aid the alignment of the mouse and the adjustment of the focal plane in the mouse retina for the STOC-T image. We extract pulsatile blood flow frequency and other hemodynamic parameters from multiple retinal and choroidal vessels from structural-only OCT images, highlighting the prospects of STOC-T for monitoring retinal hemodynamics in a simple way.
We developed and applied Spatio-Temporal Optical Coherence Tomography (STOC-T), which supported by computational aberration correction enables high resolution imaging of the human and mouse retina in vivo.
We present a novel mouse eye imaging system based on the Spatio-Temporal Optical Coherence Tomography (STOC-T) technique capable of acquiring structural image of a mouse retina at a volumetric rate of 112 Hz. A fundus camera and white light illumination aid the alignment of the mouse and the adjustment of the focal plane in the mouse retina for the STOC-T image. The fundus camera is calibrated so that when the white-light image of the mouse eye fundus appears in focus after the appropriate gel thickness is selected for a given mouse and bi-concave lens, the corresponding near infrared STOC-T image of the photoreceptor layer is also in focus, albeit with minor shifts. We present images of retinal and choroidal tissue from a B6 albino wild type mouse after the focal plane adjustment with richness of details.
We report on a novel mice imaging system based on the Spatio-Temporal Optical Coherence Tomography (STOC-T)
technique. The contribution describes the translation of the STOC-T technique, initially developed for human eye imaging, into the field of experimental small animal imaging. We present images of retinal and choroidal tissue from a B6 albino wild type mouse acquired at a volumetric rate of 112 Hz.
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