The exercise-induced fatigue eliminated by Chinese medicine offers advantages including good efficiency and smaller
side-effects, however, the exact mechanisms have not been classified. A lot of literatures indicated the cytosolic free
Ca2+ concentrations of skeletal muscle cells increased significantly during exercise-induced fatigue. This study is aimed
to establish a rat skeletal muscle cell model of exercise-induced fatigue. We applied cytoplasmic Ca2+ fluorescence
imaging techniques to study the molecular mechanisms of exercise-induced fatigue eliminated by Chinese medicine
ginseng extract. In our research, the muscle tissues from the newborn 3 days rats were taken out and digested into cells.
The cells were randomly divided into the ginseng extract group and the control group. The cells from the two groups
were cultured in the medium respectively added 2mg/ml ginseng extract and 2mg/ml D-hanks solution. After
differentiating into myotubes, the two groups of cells treated with a fluorescent probe Fluo-3 AM were put on the
confocal microscope and the fluorescence intensity of cells pre- and post- stimulation with dexamethasone were
detected. It was found that cytoplasmic Ca2+ concentrations of the two groups of cells both increased post-stimulation,
however, the increasing amplitude of fluorescence intensity of the ginseng extract group was significantly lower than
that of the control group. In conclusion, stimulating the cells with dexamethasone is a kind of workable cell models of
exercise-induced fatigue, and the molecular mechanisms of exercise-induced fatigue eliminated by ginseng extract may
be connected to regulatating cytosolic free Ca2+ concentrations.
To assess modulation of laser on erythrocyte permeability and deformability via cell morphology changes, healthy
human echinocytes with shrinking size and high plasmic viscosity due to cellular dehydration were treated with 1 mW, 2
mW, 3 mW, and 5 mW laser power exposure respectively. Image analyzing system on single intact erythrocyte was
applied for measuring comprehensive cell morphological parameters (surface area, external membrane perimeter, circle
index and elongation index) that were determined by the modulation of erythrocyte water permeability and deformability
to detect relationship between erythrocyte water permeability alteration and deformability. Our preliminary experiment
showed that exposure under light dose of 5 mW for 5 min could induce more active erythrocyte swelling and
deformation. water channel aquaporin-1(AQP-1) was inhibited by the incubation of HgCl2 in the presence and absence of
5 mW laser irradiation. The result suggested that osmotic water permeability is a primary factor in the procedure of
erythrocyte deformability. In addition, no modulation of laser(5mW) on erythrocyte deformability had been found when
the echinocytes were cultured with GDP-β-S (G protein inhibitor).
The aim of this study was to test human erythrocyte deformability with the exposure of erythrocyte from apoplexy patient and other ischemia diseases, contracted with normal donors' blood sample, and the doses-effect of Low-power He_Ne laser in vitro were discussed. Fresh blood sample from adult health donors and patients with different diseases such as apoplexy, diabetes, heart block etc in emergency department were collected and divided into different groups in which there were no less than 6 persons. Fresh human blood samples were irradiated with a He-Ne laser (Lamba=632.8nm), power output around 4.5MW, 9MW, 15mW, and 18mW, et al., exposure time from 7.5min, 15min, and 30min, operating in continuous wave. Measurements of human erythrocyte deformability were taken. Erythrocyte deformability appearance shown some different in the health contracted group and the other ischemia disease group. Some notice difference also shown among some disease group with nonirradiation and the same disease group with laser irradiation. The dose-effects of He-Ne laser therapy was discussed on the further research on the erythrocyte deformability of blood sample from patients with apoplexy disease treated with He-Ne laser at different doses, and a certain optimal doses which could take a beneficial effect in clinic were speculated on. This study revealed that the He-Ne laser have some different effects on erythrocyte deformability in vitro, which were related with the disease condition, red cell state, and outpower-doses, et al closely.
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