Dr. Zhongming Li Profile

Dr. Zhongming Li

at Koch Institute for Integrative Cancer Research

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Proceedings Article | 20 August 2015 Presentation + Paper

Super-resolution imaging with mid-IR photothermal microscopy on the single particle level

Zhongming Li, Masaru Kuno, Gregory Hartland

Proceedings Volume 9549, 954912 (2015) https://doi.org/10.1117/12.2188337

KEYWORDS: Mid-IR, Particles, Refractive index, Super resolution, Sensors, Microscopy, Spatial resolution, Absorption, Diffusion, Infrared radiation

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Photothermal microscopy has achieved single molecule sensitivity. However, the analytes are usually restricted to be natural absorbers in the visible light region. Mid-infrared (MIR) imaging, on the other hand, provides a wealth of information, but encounters difficulties of diffraction-limited spatial resolution and scarcity of ideal detectors. Here we present Mid-IR photothermal heterodyne imaging (MIR-PHI) microscopy as a high sensitivity, super-resolution mid-IR imaging technique. In MIR-PHI, a tunable Mid- IR pulsed laser at 150 kHz is used to excite a micron sized particle. Energy relaxation creates a temperature gradient around the particle, changing the refractive index of the surrounding solvent and creating a thermal lens. A collinear, counter propagating probe beam (a 532 nm CW laser) is modified by the thermal lens and generates a super-resolution photothermal image. We studied 1.1 μm polystyrene beads at the single particle level using this technique. Various solvents with different heat capacities and refractive indices are tested for the best image contrast. The wide applicability and potentially high sensitivity of this technique make it promising for biological imaging and identification.

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