Bacterial infections inside the heart, called infective endocarditis, result in high mortality. The bacteria encase themselves within a biofilm, which shields them from treatment by drugs or devices, and makes it challenging to diagnose and confirm infection, despite technological advances. In this study, we investigate the use of optical coherence microscopy (OCM), a non-invasive imaging modality, as a potential tool to visualize biofilms on heart valves. Biofilms were grown on porcine heart valves in human plasma using clinical isolates of Staphylococcus aureus or Streptococcus gordonii. S. aureus biofilms were treated with a fibrinolytic to degrade and remove biofilms. Valves were imaged before and after biofilm growth using OCM followed by subsequent confocal laser scanning microscopy using fluorescent staining. The resolutions and imaging areas of the two microscopes were matched. A comparative analysis of the two techniques showed that OCM can accurately differentiate between areas with and without biofilm. Our findings highlight OCM as a tool for non-contact, label free imaging that can provide key morphological information for infection diagnosis and therapy guidance.
In this study, we demonstrate a 12x36 mm motorized capsule for OCT imaging of the esophagus. The capsule produces unobstructed images by using a distal reflector design, thus avoiding shadow caused by the motor wires. The motor synchronous control enables three working modes: circumferential imaging, angular sector imaging and accurate beam positioning. Distortion artifacts shown in the sector imaging were found to be induced by velocity changes of the motor. We specifically characterized the motor speed and found a symmetric and repeatable behavior during sector scanning. Resampling of the sector images A-lines was carried out to achieve uniform angular spacing according to the measured speed profile. Also, distortion between consecutive sector frames was corrected using image registration to achieve stable imaging.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.