Laser speckle contrast imaging (LSCI) is a contrast-free optical method offering quantitative assessment of superficial blood vessel vascularity. Its clinical significance is evident in intraoperative scenarios, particularly for assessing parathyroid gland viability in thyroid surgery. Despite notable sensitivity and specificity, conventional overhead LSCI methods, characterized by bulky designs, present integration challenges in surgical procedures. To address these limitations, we developed a miniaturized handheld LSCI probe, enhancing portability and maneuverability. A validation assessment compared the handheld system with an overhead LSCI setup for detecting vascularity in parathyroid glands in parathyroidectomy surgeries. Results from comparisons with the established overhead LSCI system, combined with evaluations by an experienced surgeon, underscore the handheld LSCI system's effectiveness in human parathyroid gland vascularity classification. This study's findings offer valuable insights into the performance and clinical utility of the newly developed miniaturized handheld LSCI system.
Otitis media (OM) is a common disease of the middle ear, with 80% of children experiencing an infection before age three. Diagnostic methods rely on interpretation of symptoms from an otoscope, which help physicians visualize the eardrum. To provide precise structural and biochemical information, a prototype non-contact multimodal Raman spectroscopy (RS) and optical coherence tomography (OCT) system and handheld probe were created. Observation of in vitro physiologically-relevant ear models and comparison to in vivo scans from pediatric subjects presenting with OM detail application-specific development. Design challenges for clinical use, including maximum permissible exposure and physical size constraints, are presented.
Otitis media or middle-ear infection is a widespread bacterial/viral disease. Antibiotic-resistant bacteria within biofilms emerge during chronic ear infections and are challenging to treat. We explored Raman spectroscopy (RS) and Optical Coherence Tomography (OCT) to identify and compare unique spectroscopic and microstructural features from primary otopathogenic bacteria in colony, planktonic, and biofilm forms, in vitro. RS was utilized to identify biochemical fingerprints and OCT was used to generate depth-resolved 2D and 3D images to compare refractive indices and optical attenuation coefficients. A combined RS-OCT system will enable real-time visualization and diagnosis of bacterial OM at the point-of-care.
Oropharyngeal squamous cell carcinoma (OPSCC) which refers to the cancer of the back of the throat, including the base of tongue and tonsils, has rapidly increased the past several decades and if undiagnosed, the tumors metastasize leading to many complications and decreased survival. In this study, Raman spectroscopy (RS) in combination with data classification algorithms was used to examine tonsil specimens (normal, benign, and malignant) to determine if RS could serve as a viable tool for real-time sensitive detection of OPSCC and ultimately other HPV-linked cancers.
Otitis media (OM) is a group of inflammatory diseases of the middle ear and is the most frequent cause of physician visits and antibiotic prescriptions for children. Current diagnostic methods do not differentiate acute otitis media from otitis media with effusion, as both types of OM share many symptomatic features. A cohort of around 50 patients undergoing myringotomy were enrolled in this study, where Raman spectroscopy (RS) allowed non-invasive determination of OM subtype. Here we demonstrate the potential for RS to probe OM infection status in pediatric patients, to guide treatment protocol for ear infections and limit over-prescription of antibiotics.
KEYWORDS: Tumors, Breast cancer, Mammography, Phototherapy, 3D modeling, Tumor growth modeling, Cancer, Resistance, 3D image processing, Electron transport
Innate differences among breast cancer phenotypes are often studied from the perspective of single protein expressions or by fluorescent imaging using molecular tags. While these techniques can offer useful insights into breast cancer taxonomy, they do not easily translate to clinical care. Nonlinear optical microscopy has revolutionized our ability to study biochemical processes, as it offers a label-free approach to study differences in cancer phenotypes that may provide insight into factors affecting prognosis and treatment strategies. The endogenous chemical specificity offered by nonlinear imaging modalities like Stimulated Raman Scattering (SRS) and Multiphoton Fluorescence (MPF) are attractive alternatives to fluorescent imaging to study intracellular biochemistry. Here we utilize a multimodal imaging platform to characterize lipogenesis in HER2+ cell lines through SRS and metabolic activity by MPF of NADH/FAD to investigate unique signals related to HER2 status.
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