Mr. Xiangpeng Pan Profile

Xiangpeng Pan

at Nanjing Univ of Science and Technology

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Proceedings Article | 19 November 2019 Paper

Video-rate isotropic quantitative differential phase contrast microscopy based on color-multiplexed annular illumination

Yao Fan, Jiasong Sun, Qian Chen, Xiangpeng Pan, Chao Zuo

Proceedings Volume 11186, 111860F (2019) https://doi.org/10.1117/12.2537482

KEYWORDS: Phase contrast, Microscopy, Signal to noise ratio, Phase transfer function, Cameras, Temporal resolution, Phase imaging, Modulation, LCDs, Objectives

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Differential phase contrast microscopy (DPC) provides high-resolution quantitative phase distribution of thin transparent samples under multi-axis asymmetric illuminations. Typically, illumination in DPC microscopic systems is designed with 2-axis half-circle amplitude patterns, which, however, reduce the temporal resolution of DPC, precluding observation of high-speed phenomenon. Efforts have been made to achieve video-rate DPC by using tri-mode illumination or adding multi-colored filter. However, the frequency responses of the PTFs has not been improved, leading to poor phase contrast and signal-to-noise ratio (SNR) for phase reconstruction. We present a video-rate isotropic quantitative phase imaging (QPI) method based on color-multiplexed differential phase contrast (DPC). In our method, the illumination source is modulated by an LCD into an annular color-multiplexed pattern matching the numerical aperture of the objective precisely to maximize the frequency response for both low and high frequencies (from 0 to 2NA_obj). In addition, we propose an alternating illumination scheme to provide a perfectly circularly symmetrical phase transfer function (PTF), achieving isotropic imaging resolution and signal-to-noise ratio (SNR). A color camera records the light transmitted through the specimen, and three monochromatic intensity images at each color channel are then separated and utilized to recover the phase of the specimen. We present the derivation, implementation, simulation and experimental results demonstrating that our method accomplishes high resolution, noise-robustness and reconstruction accuracy at camera-limited frame rates.

Proceedings Article | 16 October 2019 Paper

Isotropic quantitative phase imaging with optimal differential phase contrast illumination scheme

Yao Fan, Jiasong Sun, Qian Chen, Xiangpeng Pan, Chao Zuo

Proceedings Volume 11205, 112050J (2019) https://doi.org/10.1117/12.2547850

KEYWORDS: Phase transfer function, Phase contrast, Phase imaging, Image resolution, LCDs, Biomedical optics, Microscopy

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Quantitative phase imaging (QPI), which provides unique imaging capabilities for optical thickness variation of living cells and tissues without the need for specific staining or exogenous contrast agents (e.g., dyes or fluorophores), has emerged as an invaluable optical tool for biomedical research. Differential phase contrast (DPC) is the most promising QPI approach to high resolution label-free cellular dynamic imaging because of its advantages of higher imaging efficiency, higher accuracy, and higher stability. Typically, illuminations in DPC systems are designed with 2-axis half-circle amplitude patterns, which however results in a non-isotropic phase transfer function (PTF). Furthermore, the frequency responses of the PTFs have not been fully optimized, leading to suboptimal phase contrast and signal-to-noise ratio (SNR) for phase reconstruction. In this paper, we derive the optimal illumination scheme to maximize the PTF response for both low and high frequencies (from 0 to 2NA_obj), and meanwhile achieve perfectly isotropic PTF with only 2-axis intensity measurements. We present the theoretical analysis, simulations, and experimental results demonstrating that our optimal illumination scheme is a simple, efficient, and stable approach for label-free quantitative cell imaging with subcellular resolution.

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