Myopia is a significant cause of visual impairment which may lead to many complications. Though the mechanism of myopia is not clear, the changes of the biological parameters and the optical performance of eyes were suggested as a key mechanism. In order to investigate the mechanism of myopia, in this paper, the time serial evaluation of biological parameters was performed and the optical performance of myopic model mouse eyes were evaluated by using optical coherence tomography (OCT) and ZEMAX software. The biological parameters were compared, and the optical performance of mouse eyes were evaluated in the myopic model mouse eyes, normal control mouse eyes and atropine-treated mouse eyes. The correlation analysis of the statistics indicated that the development of myopia was significantly related to the corneal curvature radius, center corneal thickness, vitreous depth and axial length (P<0.05). The analysis of biological parameters proved that the peripheral RMS wavefront aberration of eyes played more crucial roles in the development of myopia than the center parameters, and the degree of myopia would be proofed by the hyperopic wavefront aberration. The myopic model mouse eyes had larger corneal curvature radius, thicker center corneal thickness, deeper vitreous depth and longer axial length than normal mouse eyes. The experimental results of atropine-treated eyes suggested that atropine could relieve myopia and eye enlargement via a nonaccommodative mechanism. This study provides a new method for evaluations of the optical performance of eyes and the study of myopia.
Optical coherence tomography (OCT) is an indispensable diagnostic tool in ophthalmology which can provide crosssectional anatomic and functional information of the eyes. For analysis of the optical imaging performance of the eye, the ZEMAX ray tracing software can be used to establish the refractive model and to simulate the light spot and wavefront in ocular fundus. In our study, by combination of OCT and ray tracing technique, the imaging performance of mouse eye was evaluated and the central and peripheral image qualities were quantitatively analyzed. The whole mouse eyes were imaged by a customized OCT system and the OCT images were corrected for distortions caused by the refraction of light on the ocular surface. Parameters of mouse eye, both at central and peripheral areas, including corneal thickness, radius of curvature, lens thickness, etc., were then measured. Finally, the imaging performance of the ophthalmology system was evaluated by using ZEMAX software for ray tracing. The light spot size, the defocus blur parameter and the wavefront aberration were quantitatively evaluated. Our study may provide a method for research on myopia and hyperopia.
Optical coherence tomography (OCT) is a noninvasive, non-contact imaging technique, which is an indispensable diagnostic tool in ophthalmology. However, light attenuation in opaque tissues of the eye limits the imaging depth of OCT. In this study, the imaging depth of rabbit eye in vitro was extended by reduction of multiple scattering by using tissue optical clearing (TOC) agents. Results showed that with the application of glycerol in anterior segment, boundaries of ciliary muscle could be obviously differentiated. For posterior segment, the retina, the choroid, the sclera and even the eye container were also clearly imaged. All the physiology change by glycerol was recovered by a direct wash of saline in a short time.
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