Versatile and cost-effective multi-wavelength excitation and multispectral-emission frequency-domain fluorescence lifetime imaging system for real-time biomedical and clinical applications

Michael Serafino; Javier A. Jo

doi:10.1117/12.2608927

2 March 2022 Versatile and cost-effective multi-wavelength excitation and multispectral-emission frequency-domain fluorescence lifetime imaging system for real-time biomedical and clinical applications

Michael Serafino, Javier A. Jo

Author Affiliations +

Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720F (2022) https://doi.org/10.1117/12.2608927
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We report the development of a novel point-scanning frequency-domain (FD) fluorescence lifetime imaging (FLIM) system. The system, which was customized for label-free metabolic and biochemical imaging of oral epithelial tissue, is capable of simultaneous excitation at 375nm and 445nm, emission detection across 4 spectral bands, real-time fluorescence modulation and phase lifetime calculation, and low-cost data acquisition implementation using an Abaco FMC104 digitizer, Xilinx Zedboard FPGA, and Python software on the host computer. In this work we discuss our challenges and workflow with the goal of helping fellow researchers develop versatile and cost-effective FLIM systems for biomedical and clinical imaging applications.

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Citation Download Citation

Michael Serafino and Javier A. Jo "Versatile and cost-effective multi-wavelength excitation and multispectral-emission frequency-domain fluorescence lifetime imaging system for real-time biomedical and clinical applications", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720F (2 March 2022); https://doi.org/10.1117/12.2608927

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KEYWORDS

Fluorescence lifetime imaging

Imaging systems

Field programmable gate arrays

Biomedical optics

Modulation

Software development

Phase shift keying

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