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20 May 2022 Focus plane estimation in digital holographic microscopy
Corinne Fournier, Dylan Brault, Thomas Olivier, Nicolas Faure, Sophie Dixneuf, Louis Thibon, Loic Mees, Loïc Denis
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Proceedings Volume PC12136, Unconventional Optical Imaging III; PC1213615 (2022) https://doi.org/10.1117/12.2623071
Event: SPIE Photonics Europe, 2022, Strasbourg, France
Abstract
We present a new method to achieve digital autofocus in holography. This method relies on the insertion of calibrated beads into the studied sample. Reconstructing the position and the radius of the beads using Inverse Problems Approach, based on Mie Model, makes it possible to accurately locate the slide on which the sampled is placed. Numerical focusing can then be performed using the standard backpropagation method or regularized reconstruction. Because the reconstruction plane can be chosen objectively with respect to the position of the slide, this numerical autofocus is reproducible whatever is the type of the observed biological sample.
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Corinne Fournier, Dylan Brault, Thomas Olivier, Nicolas Faure, Sophie Dixneuf, Louis Thibon, Loic Mees, and Loïc Denis "Focus plane estimation in digital holographic microscopy", Proc. SPIE PC12136, Unconventional Optical Imaging III, PC1213615 (20 May 2022); https://doi.org/10.1117/12.2623071
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KEYWORDS
Digital holography
Holography
Microscopy
Inverse optics
Inverse problems
Particles
Calibration
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