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16 March 2023 High-speed interferometric scattering confocal microscopy for label-free live cell imaging (Conference Presentation)
Tsai-Ying Wu, Yi-Teng Hsiao, Bo-Kuan Wu, Shi-Wei Chu, Chia-Lung Hsieh
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Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC123910V (2023) https://doi.org/10.1117/12.2648647
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We demonstrate a high-speed confocal-based interferometric scattering (iSCAT) microscopy for label-free live cell imaging. Using a spinning disk scanning unit, our wide-field iSCAT confocal microscope reaches a high image acquisition rate of 1,000 frames per second. Nano-sized objects, as small as 10 nm gold nanoparticles, are directly visualized by the iSCAT confocal microscope in scattering. With the high spatiotemporal resolutions and the high sensitivity, we explore the complex organization and dynamics in live cells.
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Tsai-Ying Wu, Yi-Teng Hsiao, Bo-Kuan Wu, Shi-Wei Chu, and Chia-Lung Hsieh "High-speed interferometric scattering confocal microscopy for label-free live cell imaging (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC123910V (16 March 2023); https://doi.org/10.1117/12.2648647
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KEYWORDS
Scattering
Confocal microscopy
Interferometry
Light scattering
Live cell imaging
Microscopy
Microscopes
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