Assessing in vivo label-free fluorescence lifetime Imaging sensitivity to low-grade colon inflammation

Alba Alfonso García; Jee-Yon Lee; Alex Condon; Xiangnan Zhou; Julien Bec; Andreas J. Baumler; Laura Marcu

doi:10.1117/12.3002008

13 March 2024 Assessing in vivo label-free fluorescence lifetime Imaging sensitivity to low-grade colon inflammation

Alba Alfonso García, Jee-Yon Lee, Alex Condon, Xiangnan Zhou, Julien Bec, Andreas J. Baumler, Laura Marcu

Proceedings Volume PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024; PC1282206 (2024) https://doi.org/10.1117/12.3002008
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

This study assesses the sensitivity of label-free fluorescence lifetime imaging (FLIm) for detecting low-grade gastrointestinal inflammation in mice. A FLIm probe was developed for in vivo colon imaging, enabling complete colon scanning. Using the probe, low-grade inflammation induced by streptomycin was imaged, showing decreased fluorescence lifetime at wavelengths related to metabolic activity, indicating a shift to glycolytic metabolism in inflamed tissue. The potential of validating FLIm maps with spatial transcriptomics was explored. These methodologies provide a basis for further experimentation to establish FLIm as a tool to quantify colon epithelial metabolism over time and its relevance to monitor colorectal inflammatory disease.

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Citation Download Citation

Alba Alfonso García, Jee-Yon Lee, Alex Condon, Xiangnan Zhou, Julien Bec, Andreas J. Baumler, and Laura Marcu "Assessing in vivo label-free fluorescence lifetime Imaging sensitivity to low-grade colon inflammation", Proc. SPIE PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, PC1282206 (13 March 2024); https://doi.org/10.1117/12.3002008

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KEYWORDS

Colon

Inflammation

Fluorescence lifetime imaging

In vivo imaging

Tissues

Mode conditioning cables

Micro optics

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