In vivo and ex vivo differentiation between tumor and healthy tissue with a novel macroscale time-domain fluorescence lifetime imaging system: preclinical results

Simone Janssen; Thomas Van den Dries; Marcus Stroet; Sevada Sahakian; Thomas Lapauw; Maarten Kuijk; Hilde De Rooster; Hans Ingelberts; Sophie Hernot

doi:10.1117/12.3002712

13 March 2024 In vivo and ex vivo differentiation between tumor and healthy tissue with a novel macroscale time-domain fluorescence lifetime imaging system: preclinical results

Simone Janssen, Thomas Van den Dries, Marcus Stroet, Sevada Sahakian, Thomas Lapauw, Maarten Kuijk, Hilde De Rooster, Hans Ingelberts, Sophie Hernot

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Proceedings Volume PC12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X; PC128250Q (2024) https://doi.org/10.1117/12.3002712
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Recent studies demonstrated the added-value of fluorescence lifetime (FLT) imaging in tumor identification to intensity-based imaging. The tauCAM is a novel macroscale FLT imaging system that detects the FLT of a fluorophore by measuring fluorescence in the time-domain. FLT images are made simultaneously to fluorescence intensity- and grayscale reflectance images, under ambient illumination. In vivo imaging in subcutaneous tumor bearing-mice revealed that FLT imaging with the tauCAM provides additional information on environmental parameters between tumor and healthy tissue, for tracers sensitive to their physiological environment. This research emphasizes the importance of fluorophore selection for future (pre-) clinical FLT imaging trials.

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Simone Janssen, Thomas Van den Dries, Marcus Stroet, Sevada Sahakian, Thomas Lapauw, Maarten Kuijk, Hilde De Rooster, Hans Ingelberts, and Sophie Hernot "In vivo and ex vivo differentiation between tumor and healthy tissue with a novel macroscale time-domain fluorescence lifetime imaging system: preclinical results", Proc. SPIE PC12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X, PC128250Q (13 March 2024); https://doi.org/10.1117/12.3002712

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KEYWORDS

Tumors

Tissues

Imaging systems

In vivo imaging

Fluorescence lifetime imaging

Fluorescence imaging

Fluorescence intensity

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