Pathologist evaluation of brightfield microscopy images of H&E-stained tissues is time-consuming and labor-intensive, preventing intraoperative feedback to surgeons. Combined ultraviolet reflectance and photoacoustic remote sensing (PARS) microscopy was recently introduced, providing a label-free way to obtain cell nuclei and cytoplasm images. Additionally, utilizing deep learning and fast voice-coil scanning, maximally realistic virtual histology can be rapidly generated with this technique. Here, we introduce combined ultraviolet confocal reflectance, autofluorescence, and PARS microscopy, utilizing a single 266 nm excitation beam. This system now allows us to generate sectioned virtual histology in thick tissues well suited to the intraoperative setting.
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