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13 March 2024 Bringing time-resolved fluorescence microscopy to a broader research community
Felix Koberling, Fabio Barachati, Marcelle Koenig, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, Rainer Erdmann
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Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC1284708 (2024) https://doi.org/10.1117/12.3007783
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
PicoQuant`s new confocal microscope Luminosa combines state-of-the-art hardware with cutting edge software to deliver high quality data while simplifying daily operation. Luminosa’s rapidFLIM hardware can record several frames per second, which the software handles with a novel dynamic binning format. In combination with GPU-accelerated algorithms, this enables high-speed automated analysis of FLIM images. We demonstrate the combination of PicoQuant’s latest multi-channel TCSPC device and a cooled high-performance 23-pixel SPAD-array developed jointly with Pi Imaging Technologies for time-resolved confocal image scanning microscopy (FLIM-ISM). We also discuss how advanced data processing can be applied to FLIM-ISM for additional performance gains. For certain applications widefield imaging offers unique advantages. Here we show how the combination of a powerful PicoQuant laser and the novel SPAD512S camera from Pi Imaging Technology facilitates time-resolved widefield imaging. We benchmark the camera’s performance, demonstrating video rate acquisition speeds with minimal photobleaching.
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Felix Koberling, Fabio Barachati, Marcelle Koenig, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, and Rainer Erdmann "Bringing time-resolved fluorescence microscopy to a broader research community", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC1284708 (13 March 2024); https://doi.org/10.1117/12.3007783
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KEYWORDS
Confocal microscopy
Fluorescence lifetime imaging
Fluorescence microscopy
Cameras
Imaging technologies
Picosecond phenomena
Sampling rates
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