Biosensors for detecting stress in developing embryos

Malcolm S. Purdey; Avishkar Saini; Hanna J. McLennan; Benjamin J. Pullen; Erik P. Schartner; Melanie L. Sutton-McDowall; Jeremy G. Thompson; Tanya M. Monro; Stephen J. Nicholls; Andrew D. Abell

doi:10.1117/12.2244645

9 December 2016 Biosensors for detecting stress in developing embryos

Malcolm S. Purdey, Avishkar Saini, Hanna J. McLennan, Benjamin J. Pullen, Erik P. Schartner, Melanie L. Sutton-McDowall, Jeremy G. Thompson, Tanya M. Monro, Stephen J. Nicholls, Andrew D. Abell

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Proceedings Volume 10013, SPIE BioPhotonics Australasia; 100130B (2016) https://doi.org/10.1117/12.2244645
Event: SPIE BioPhotonics Australasia, 2016, Adelaide, Australia

Abstract

Reactive Oxygen Species (ROS) cause DNA damage and defective function in sperm and also affects the developmental competence of embryos. It is therefore critical to monitor ROS in sperm, oocytes and developing embryos. In particular, hydrogen peroxide (H₂O₂) is a ROS important to normal cell function and signalling as well as its role in oxidative stress. Here we report the development of a fluorescent sensor for H₂O₂ using carboxyperoxyfluor-1 (CPF1) in solution and attached to a glass slide or multi-mode optical fibre. CPF1 increases in fluorescence upon reaction with H₂O₂ to non-invasively detect H₂O₂ near developing embryos. These probes are constructed by immobilising CPF1 to the optical fibre tip a polyacrylamide layer. Also reported is a new dual optical fibre sensor for detecting both H₂O₂ and pH that is functional at biologically concentrations of H₂O₂ and can sense pH to 0.1 units. This research shows promise for the use of optical fibre sensors for monitoring the health of developing embryos. Furthermore, these sensors are applicable for use beyond embryos such as detecting stress in endothelial cells involved in cardiovascular dysfunction.

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Malcolm S. Purdey, Avishkar Saini, Hanna J. McLennan, Benjamin J. Pullen, Erik P. Schartner, Melanie L. Sutton-McDowall, Jeremy G. Thompson, Tanya M. Monro, Stephen J. Nicholls, and Andrew D. Abell "Biosensors for detecting stress in developing embryos", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100130B (9 December 2016); https://doi.org/10.1117/12.2244645

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KEYWORDS

Optical fibers

Sensors

Biosensors

Detector development

Luminescence

Biomedical optics

Hydrogen

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