Imaging mouse cerebellum with serial optical coherence scanner (Conference Presentation)

Chao J. Liu; Kristen Williams; Harry Orr; Akkin Taner

doi:10.1117/12.2251532

19 April 2017 Imaging mouse cerebellum with serial optical coherence scanner (Conference Presentation)

Chao J. Liu, Kristen Williams, Harry Orr, Akkin Taner

Proceedings Volume 10051, Neural Imaging and Sensing; 1005106 (2017) https://doi.org/10.1117/12.2251532
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We present the serial optical coherence scanner (SOCS), which consists of a polarization sensitive optical coherence tomography and a vibratome with associated controls for serial imaging, to visualize the cerebellum and adjacent brainstem of mouse. The cerebellar cortical layers and white matter are distinguished by using intrinsic optical contrasts. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and adjacent brainstem. The optical system, which has ~5.5 μm axial resolution, utilizes a scan lens or a water-immersion microscope objective resulting in 10 μm or 4 μm lateral resolution, respectively. The large-scale brain imaging at high resolution requires an efficient way to collect large datasets. It is important to improve the SOCS system to deal with large-scale and large number of samples in a reasonable time. The imaging and slicing procedure for a section took about 4 minutes due to a low speed of the vibratome blade to maintain slicing quality. SOCS has potential to investigate pathological changes and monitor the effects of therapeutic drugs in cerebellar diseases such as spinocerebellar ataxia 1 (SCA1). The SCA1 is a neurodegenerative disease characterized by atrophy and eventual loss of Purkinje cells from the cerebellar cortex, and the optical contrasts provided by SOCS is being evaluated for biomarkers of the disease.

Conference Presentation

Citation Download Citation

Chao J. Liu, Kristen Williams, Harry Orr, and Akkin Taner "Imaging mouse cerebellum with serial optical coherence scanner (Conference Presentation)", Proc. SPIE 10051, Neural Imaging and Sensing, 1005106 (19 April 2017); https://doi.org/10.1117/12.2251532

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Cerebellum

System on a chip

Coherence (optics)

Scanners

Brain mapping

Control systems

Optical coherence tomography

Show All Keywords

Keywords/Phrases

Search In:

Publication Years