Raman spectroscopy for discrimination of neural progenitor cells and their lineages (Conference Presentation)

Keren Chen; William Ong; Sing Yian Chew; Quan Liu

doi:10.1117/12.2250146

18 April 2017 Raman spectroscopy for discrimination of neural progenitor cells and their lineages (Conference Presentation)

Keren Chen, William Ong, Sing Yian Chew, Quan Liu

Author Affiliations +

Proceedings Volume 10054, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XV; 100540B (2017) https://doi.org/10.1117/12.2250146
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Neurological diseases are one of the leading causes of adult disability and they are estimated to cause more deaths than cancer in the elderly population by 2040. Stem cell therapy has shown great potential in treating neurological diseases. However, before cell therapy can be widely adopted in the long term, a number of challenges need to be addressed, including the fundamental research about cellular development of neural progenitor cells. To facilitate the fundamental research of neural progenitor cells, many methods have been developed to identify neural progenitor cells. Although great progress has been made, there is still lack of an effective method to achieve fast, label-free and noninvasive differentiation of neural progenitor cells and their lineages. As a fast, label-free and noninvasive technique, spontaneous Raman spectroscopy has been conducted to characterize many types of stem cells including neural stem cells. However, to our best knowledge, it has not been studied for the discrimination of neural progenitor cells from specific lineages. Here we report the differentiation of neural progenitor cell from their lineages including astrocytes, oligodendrocytes and neurons using spontaneous Raman spectroscopy. Moreover, we also evaluate the influence of system parameters during spectral acquisition on the quality of measured Raman spectra and the accuracy of classification using the spectra, which yield a set of optimal system parameters facilitating future studies.

Conference Presentation

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Keren Chen, William Ong, Sing Yian Chew, and Quan Liu "Raman spectroscopy for discrimination of neural progenitor cells and their lineages (Conference Presentation)", Proc. SPIE 10054, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XV, 100540B (18 April 2017); https://doi.org/10.1117/12.2250146

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KEYWORDS

Raman spectroscopy

Stem cells

Cancer

Classification systems

Neurons

Quality measurement

Biomedical optics

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