A portable fluorescent sensing system using multiple LEDs

Young-Ho Shin; Jonathan Z. Barnett; M. Teresa Gutierrez-Wing; Kelly A. Rusch; Jin-Woo Choi

doi:10.1117/12.2261292

28 February 2017 A portable fluorescent sensing system using multiple LEDs

Young-Ho Shin, Jonathan Z. Barnett, M. Teresa Gutierrez-Wing, Kelly A. Rusch, Jin-Woo Choi

Proceedings Volume 10061, Microfluidics, BioMEMS, and Medical Microsystems XV; 100610M (2017) https://doi.org/10.1117/12.2261292
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

This paper presents a portable fluorescent sensing system that utilizes different light emitting diode (LED) excitation lights for multiple target detection. In order to identify different analytes, three different wavelengths (385 nm, 448 nm, and 590 nm) of excitation light emitting diodes were used to selectively stimulate the target analytes. A highly sensitive silicon photomultiplier (SiPM) was used to detect corresponding fluorescent signals from each analyte. Based on the unique fluorescent response of each analyte, it is possible to simultaneously differentiate one analyte from the other in a mixture of target analytes. A portable system was designed and fabricated consisting of a display module, battery, data storage card, and sample loading tray into a compact 3D-printed jig. The portable sensor system was demonstrated for quantification and differentiation of microalgae (Chlorella vulgaris) and cyanobacteria (Spirulina) by measuring fluorescent responses of chlorophyll a in microalgae and phycocyanin in cyanobacteria. Obtained results suggest that the developed portable sensor system could be used as a generic fluorescence sensor platform for on-site detection of multiple analytes of interest.

Citation Download Citation

Young-Ho Shin, Jonathan Z. Barnett, M. Teresa Gutierrez-Wing, Kelly A. Rusch, and Jin-Woo Choi "A portable fluorescent sensing system using multiple LEDs", Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 100610M (28 February 2017); https://doi.org/10.1117/12.2261292

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