Ultra-fast 3D scanning and holographic illumination in non-linear microscopy using acousto-optic deflectors

Walther Akemann; Cathie Ventalon; Jean-François Léger; Benjamin Mathieu; Stéphane Dieudonné; Baptiste Blochet; Sylvain Gigan; Laurent Bourdieu

doi:10.1117/12.2275143

18 April 2017 Ultra-fast 3D scanning and holographic illumination in non-linear microscopy using acousto-optic deflectors

Walther Akemann, Cathie Ventalon, Jean-François Léger, Benjamin Mathieu, Stéphane Dieudonné, Baptiste Blochet, Sylvain Gigan, Laurent Bourdieu

Author Affiliations +

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510L (2017) https://doi.org/10.1117/12.2275143
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

Decoding of information in the brain requires the imaging of large neuronal networks using e.g. two-photon microscopy (TPM). Fast control of the focus in 3D can be achieved with phase shaping of the light beam using acoustooptic deflectors (AODs). However, beam shaping using AODs is not straightforward because of non-stationary of acousto-optic diffraction. Here, we demonstrated a new stable AOD-based phase modulator, which operates at a rate of up to about hundred kHz. It provides opportunity for 3D scanning in TPM with the possibility to correct aberrations independently for every focus position or to achieve refocusing of scattered photons in rapidly decorrelating tissues.

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Walther Akemann, Cathie Ventalon, Jean-François Léger, Benjamin Mathieu, Stéphane Dieudonné, Baptiste Blochet, Sylvain Gigan, and Laurent Bourdieu "Ultra-fast 3D scanning and holographic illumination in non-linear microscopy using acousto-optic deflectors", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510L (18 April 2017); https://doi.org/10.1117/12.2275143

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