High-speed bioimaging with frequency-division-multiplexed fluorescence confocal microscopy

Hideharu Mikami; Jeffrey Harmon; Yasuyuki Ozeki; Keisuke Goda

doi:10.1117/12.2272924

18 April 2017 High-speed bioimaging with frequency-division-multiplexed fluorescence confocal microscopy

Hideharu Mikami, Jeffrey Harmon, Yasuyuki Ozeki, Keisuke Goda

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510M (2017) https://doi.org/10.1117/12.2272924
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

We present methods of fluorescence confocal microscopy that enable unprecedentedly high frame rate of > 10,000 fps. The methods are based on a frequency-division multiplexing technique, which was originally developed in the field of communication engineering. Specifically, we achieved a broad bandwidth (~400 MHz) of detection signals using a dual- AOD method and overcame limitations in frame rate, due to a scanning device, by using a multi-line focusing method, resulting in a significant increase in frame rate. The methods have potential biomedical applications such as observation of sub-millisecond dynamics in biological tissues, in-vivo three-dimensional imaging, and fluorescence imaging flow cytometry.

Citation Download Citation

Hideharu Mikami, Jeffrey Harmon, Yasuyuki Ozeki, and Keisuke Goda "High-speed bioimaging with frequency-division-multiplexed fluorescence confocal microscopy", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510M (18 April 2017); https://doi.org/10.1117/12.2272924

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