Labeling single cell for in-vivo study of cell fate mapping and lineage tracing

Sicong He; Jin Xu; Yi Wu; Ye Tian; Qiqi Sun; Zilong Wen; Jianan Y. Qu

doi:10.1117/12.2286819

20 February 2018 Labeling single cell for in-vivo study of cell fate mapping and lineage tracing

Sicong He, Jin Xu, Yi Wu, Ye Tian, Qiqi Sun, Zilong Wen, Jianan Y. Qu

Proceedings Volume 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI; 104970J (2018) https://doi.org/10.1117/12.2286819
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Cell fate mapping and lineage tracing are significant ways to understanding the developmental origins of biological tissues. It requires labeling individual cells and tracing the development of their progeny. We develop an infrared laser-evoked gene operator heat-shock microscope system to achieve single-cell labeling in zebrafish. With a fluorescent thermometry technique, we measure the temperature increase in zebrafish tissues induced by infrared laser and identify the optimal heat shock conditions for single-cell gene induction in different types of zebrafish cells. We use this technique to study the fate mapping of T lymphocytes and discover the distinct waves of lymphopoiesis during the zebrafish development.

Citation Download Citation

Sicong He, Jin Xu, Yi Wu, Ye Tian, Qiqi Sun, Zilong Wen, and Jianan Y. Qu "Labeling single cell for in-vivo study of cell fate mapping and lineage tracing", Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104970J (20 February 2018); https://doi.org/10.1117/12.2286819

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