Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy

Tsubasa Kobayashi; Yu-Rok Cha; Yuichi Kaji; Tetsuro Oshika; Philippe Leproux; Vincent Couderc; Hideaki Kano

doi:10.1117/12.2288136

23 February 2018 Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy

Tsubasa Kobayashi, Yu-Rok Cha, Yuichi Kaji, Tetsuro Oshika, Philippe Leproux, Vincent Couderc, Hideaki Kano

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104982J (2018) https://doi.org/10.1117/12.2288136
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Acanthamoeba keratitis is a disease in which amoebae named Acanthamoeba invade the cornea of an eye. To diagnose this disease before it becomes serious, it is important to detect the cyst state of Acanthamoeba in the early stage of infection. In the present study, we explored spectroscopic signitures of the cyst state of Acanthamoeba using multimodal nonlinear optical microscopy with the channels of multiplex coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and third harmonic generation (THG). A sharp band at around 1603 cm^-1 in the CARS (Im[χ⁽³⁾]) spectrum was found at the cyst state of Acanthamoeba, which possibly originates from ergosterol and/or 7-dehydrostigmasterol. It can be used as a maker band of Acanthamoeba for medical treatment. Keyword: Acanthamoeba keratitis, coherent anti-Stokes Raman scattering, CARS, second harmonic generation, SHG, microspectroscopy, multiphoton microscopy

Citation Download Citation

Tsubasa Kobayashi, Yu-Rok Cha, Yuichi Kaji, Tetsuro Oshika, Philippe Leproux, Vincent Couderc, and Hideaki Kano "Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104982J (23 February 2018); https://doi.org/10.1117/12.2288136

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