Conjugate adaptive optics with remote focusing in multiphoton microscopy

Xiaodong Tao; Tuwin Lam; Bingzhao Zhu; Qinggele Li; Marc R. Reinig; Joel Kubby

doi:10.1117/12.2292492

23 February 2018 Conjugate adaptive optics with remote focusing in multiphoton microscopy

Xiaodong Tao, Tuwin Lam, Bingzhao Zhu, Qinggele Li, Marc R. Reinig, Joel Kubby

Proceedings Volume 10502, Adaptive Optics and Wavefront Control for Biological Systems IV; 105020E (2018) https://doi.org/10.1117/12.2292492
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The small correction volume for conventional wavefront shaping methods limits their application in biological imaging through scattering media. In this paper, we take advantage of conjugate adaptive optics (CAO) and remote focusing (CAORF) to achieve three-dimensional (3D) scanning through a scattering layer with a single correction. Our results show that the proposed system can provide 10 times wider axial field of view compared with a conventional conjugate AO system when 16,384 segments are used on a spatial light modulator. We demonstrate two-photon imaging with CAORF through mouse skull. The fluorescent microspheres embedded under the scattering layers can be clearly observed after applying the correction.

Conference Presentation

Citation Download Citation

Xiaodong Tao, Tuwin Lam, Bingzhao Zhu, Qinggele Li, Marc R. Reinig, and Joel Kubby "Conjugate adaptive optics with remote focusing in multiphoton microscopy", Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 105020E (23 February 2018); https://doi.org/10.1117/12.2292492

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