Multicolor stimulated Raman imaging of live microalgal cells using fast wavelength-switched laser pulses (Conference Presentation)

Yuta Suzuki; Koya Kobayashi; Dinghuan Deng; Yoshifumi Wakisaka; Keisuke Goda; Yasuyuki Ozeki

doi:10.1117/12.2291189

15 March 2018 Multicolor stimulated Raman imaging of live microalgal cells using fast wavelength-switched laser pulses (Conference Presentation)

Yuta Suzuki, Koya Kobayashi, Dinghuan Deng, Yoshifumi Wakisaka, Keisuke Goda, Yasuyuki Ozeki

Author Affiliations +

Proceedings Volume 10505, High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management; 105050N (2018) https://doi.org/10.1117/12.2291189
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

High-speed label-free imaging with chemical contrast is effective for non-invasive analysis of the metabolic heterogeneity of single cells. Stimulated Raman scattering (SRS) microscopy enables high-speed label-free image acquisition with molecular vibrational specificity. While single-color SRS microscopy only acquires images at a certain vibrational frequency, multicolor SRS microscopy successively acquires SRS images at different vibrational frequencies, which then can be used to investigate the distributions of different intracellular molecules. However, its imaging speed remains an order of magnitude slower than that of single-color video-rate SRS microscopy. Previous approaches to circumvent this issue used either only two colors with limited chemical specificity or multiplex detection of SRS spectra using a photodiode array at the expense of imaging speed. Here we demonstrate high-speed four-color SRS imaging using a single photodiode by introducing fast wavelength-switched laser pulses. The fast wavelength switching is realized by the use of an optical intensity modulator as a time gate, a diffraction grating, and fiber delay lines. Using the developed system, we demonstrate motion-artifact-free multicolor SRS imaging of polymer beads and living cells. The results firmly support that our method is a powerful tool for the label-free analysis of living cells in microbiology, oncology, plant science, and medicine.

Conference Presentation

Citation Download Citation

Yuta Suzuki, Koya Kobayashi, Dinghuan Deng, Yoshifumi Wakisaka, Keisuke Goda, and Yasuyuki Ozeki "Multicolor stimulated Raman imaging of live microalgal cells using fast wavelength-switched laser pulses (Conference Presentation)", Proc. SPIE 10505, High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management, 105050N (15 March 2018); https://doi.org/10.1117/12.2291189

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Microscopy

Chemical analysis

Imaging systems

Photodiodes

Raman scattering

High speed imaging

Image acquisition

Show All Keywords

Keywords/Phrases

Search In:

Publication Years