3D imaging of theranostic nanoparticles in mice organs by means of x-ray phase contrast tomography

E. Longo; A. Bravin; F. Brun; I. Bukreeva; A. Cedola; O. De La Rochefoucauld; M. Fratini; X. Le Guevel; L. Massimi; L. Sancey; O. Tillement; P. Zeitoun

doi:10.1117/12.2293090

9 March 2018 3D imaging of theranostic nanoparticles in mice organs by means of x-ray phase contrast tomography

E. Longo, A. Bravin, F. Brun, I. Bukreeva, A. Cedola, O. De La Rochefoucauld, M. Fratini, X. Le Guevel, L. Massimi, L. Sancey, O. Tillement, P. Zeitoun

Author Affiliations +

Proceedings Volume 10573, Medical Imaging 2018: Physics of Medical Imaging; 105734I (2018) https://doi.org/10.1117/12.2293090
Event: SPIE Medical Imaging, 2018, Houston, Texas, United States

Abstract

Theranostics is an innovative research field that aims to develop high target specificity cancer treatments by administering small metal-based nanoparticles (NPs). This new generation of compounds exhibits diagnostic and therapeutic properties due to the high atomic number of their metal component. In the framework of a combined research program on low dose X-ray imaging and theranostic NPs, X-ray Phase Contrast Tomography (XPCT) was performed at ESRF using a 3 μm pixel optical system on two samples: a mouse brain bearing melanoma metastases injected with gadolinium NPs and, a mouse liver injected with gold NPs. XPCT is a non-destructive technique suitable to achieve the 3D reconstruction of a specimen and, widely used at micro-scale to detect abnormalities of the vessels, which are associated to the tumor growth or to the development of neurodegenerative diseases. Moreover, XPCT represents a promising and complementary tool to study the biodistribution of theranostic NPs in biological materials, thanks to the strong contrast with respect to soft tissues that metal-based NPs provide in radiological images. This work is relied on an original imaging approach based on the evaluation of the contrast differences between the images acquired below and above K-edge energies, as a proof of the certain localization of NPs. We will present different methods aiming to enhance the localization of NPs and a 3D map of their distribution in large volume of tissues.

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E. Longo, A. Bravin, F. Brun, I. Bukreeva, A. Cedola, O. De La Rochefoucauld, M. Fratini, X. Le Guevel, L. Massimi, L. Sancey, O. Tillement, and P. Zeitoun "3D imaging of theranostic nanoparticles in mice organs by means of x-ray phase contrast tomography", Proc. SPIE 10573, Medical Imaging 2018: Physics of Medical Imaging, 105734I (9 March 2018); https://doi.org/10.1117/12.2293090

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