Fluorescent Nucleic Acid Hybridization Methods.

D. Pinkel; J. Gray; R. Segraves; F. Waldman; B. Trask; L. C. Yu; D. Eastmond; P. Dean

doi:10.1117/12.951898

13 June 1989 Fluorescent Nucleic Acid Hybridization Methods.

D. Pinkel, J. Gray, R. Segraves, F. Waldman, B. Trask, L. C. Yu, D. Eastmond, P. Dean

Proceedings Volume 1063, New Technologies in Cytometry; (1989) https://doi.org/10.1117/12.951898
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

In the last several years a number of approaches have been developed that permit detection of nucleic acid hybridization using fluorescence techniques. These have several advantages over the previously available radioactive procedures. Among these are much higher spatial resolution, the speed with which the results are available, the reduced hazard, and the possibility of distinguishing the binding of multiple probes using different fluorochromes. Exploitation of this potential requires application of optical techniques of increasing sophistication such as development of new fluorophores, improved methods of visualizing multiple dyes in the same sample, application of image processing to extract quantitative information, and optimized optical sectioning for three dimensional reconstructions.

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D. Pinkel, J. Gray, R. Segraves, F. Waldman, B. Trask, L. C. Yu, D. Eastmond, and P. Dean "Fluorescent Nucleic Acid Hybridization Methods.", Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); https://doi.org/10.1117/12.951898

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