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18 September 2018 Laser scanning confocal microscopy using illumination beams with different polarizations in quick succession
Ranjan Kalita, S. S. Goutam Buddha, Bosanta R. Boruah
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Proceedings Volume 10772, Unconventional and Indirect Imaging, Image Reconstruction, and Wavefront Sensing 2018; 107720I (2018) https://doi.org/10.1117/12.2320176
Event: SPIE Optical Engineering + Applications, 2018, San Diego, California, United States
Abstract
The incorporation of polarization control on the illumination beam in a laser scanning confocal microscope allows extraction of the orientational information of submicroscopic features of a sample being studied. In this paper, we present the implementation of an optical arrangement that generates homogeneous as well as non-homogeneous user define polarization profiles over the cross-sectional area of a laser beam. A confocal system is built with this optical arrangement to obtain images of the same target with different polarized illumination beams such that there is considerable reduction in the time gap between two consecutive illuminations of each location of the sample.
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Ranjan Kalita, S. S. Goutam Buddha, and Bosanta R. Boruah "Laser scanning confocal microscopy using illumination beams with different polarizations in quick succession", Proc. SPIE 10772, Unconventional and Indirect Imaging, Image Reconstruction, and Wavefront Sensing 2018, 107720I (18 September 2018); https://doi.org/10.1117/12.2320176
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KEYWORDS
Polarization
Holograms
Microscopes
Confocal microscopy
Laser scanners
Image processing
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