Quantitative FRET measurement of the interaction of A1 adenosine receptors and A2A adenosine receptors in living cell

Yating Lin; Yiming Huang; Zhiwei Wu; Jianshu Xu; Ya Hu; Shusen Xie; Juqiang Lin

doi:10.1117/12.2500692

23 October 2018 Quantitative FRET measurement of the interaction of A₁ adenosine receptors and A_2A adenosine receptors in living cell

Yating Lin, Yiming Huang, Zhiwei Wu, Jianshu Xu, Ya Hu, Shusen Xie, Juqiang Lin

Author Affiliations +

Proceedings Volume 10820, Optics in Health Care and Biomedical Optics VIII; 108201V (2018) https://doi.org/10.1117/12.2500692
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

Adenosine plays important roles in the pain signal transduction by activating adenosine receptors of two subtypes of A₁ and A₂_A. In this study, FRET system based independent emission -spectral spectral spectral unmixing method (Iem-spFRET) was set up and used to measure the energy transfer from A₁R to A_2AR. The energy transfer efficiency calculated by Iem-spFRET is about 17.44%. All the above date and results demonstrate that FRET with special designed fluorescence proteins could be used to investigate the interaction between adenosine receptors.

Citation Download Citation

Yating Lin, Yiming Huang, Zhiwei Wu, Jianshu Xu, Ya Hu, Shusen Xie, and Juqiang Lin "Quantitative FRET measurement of the interaction of A₁ adenosine receptors and A_2A adenosine receptors in living cell", Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201V (23 October 2018); https://doi.org/10.1117/12.2500692

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