Miniaturization of fluorescence detection for PCR chip

Deuk-Ju Lee; Ji-Soo Hwang; Ji-Seung Park; Chan-Young Park; Hye-Jeong Song; Yu-Seop Kim; Jongwon Kim; Jong-Dae Kim

doi:10.1117/12.2509313

20 February 2019 Miniaturization of fluorescence detection for PCR chip

Deuk-Ju Lee, Ji-Soo Hwang, Ji-Seung Park, Chan-Young Park, Hye-Jeong Song, Yu-Seop Kim, Jongwon Kim, Jong-Dae Kim

Proceedings Volume 10885, Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics; 108850L (2019) https://doi.org/10.1117/12.2509313
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

In this paper, we propose a compact real time polymerase chain reaction (PCR) system for detecting fluorescence of PCR chips. Various fluorescence detection methods have been studied, but all require many optical components and optical distances. Therefore, this paper proposes a system to detect fluorescence by attaching LED and photodiode directly to reagent surface. This system is very compact and inexpensive because it requires no optical component except only excitation and emission filters. In order to optimize the performance of the proposed system, we investigated the placement of LEDs and photodiodes, the choice of filters, the signal amplification circuit, and air bubble problems during PCR amplification. Modification of the PCR protocol to obtain better results have also been studied. PCR amplification experiments on real DNA samples showed that the proposed system works well and compactness and portability are greatly increased compared to the conventional system.

Conference Presentation

Citation Download Citation

Deuk-Ju Lee, Ji-Soo Hwang, Ji-Seung Park, Chan-Young Park, Hye-Jeong Song, Yu-Seop Kim, Jongwon Kim, and Jong-Dae Kim "Miniaturization of fluorescence detection for PCR chip", Proc. SPIE 10885, Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics, 108850L (20 February 2019); https://doi.org/10.1117/12.2509313

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