Integrated confocal imaging system

Stefan Richter; Carsten Wehe; Uwe Wolf; Thomas Nobis; Lars-Christian Wittig; Philipp Huebner; Alexander Froeber; Gerhard Krampert; Dirk Thiele; Joerg Muetze; Michael Kempe

doi:10.1117/12.2514552

4 March 2019 Integrated confocal imaging system

Stefan Richter, Carsten Wehe, Uwe Wolf, Thomas Nobis, Lars-Christian Wittig, Philipp Huebner, Alexander Froeber, Gerhard Krampert, Dirk Thiele, Joerg Muetze, Michael Kempe

Author Affiliations +

Proceedings Volume 10931, MOEMS and Miniaturized Systems XVIII; 109310K (2019) https://doi.org/10.1117/12.2514552
Event: SPIE OPTO, 2019, San Francisco, California, United States

Abstract

We report on a concept of a benchtop microscope for routine applications. This concept system transfers key features of a high-end laser scanning microscope to a dedicated confocal fluorescence imaging system with appropriate footprint and reduced systems complexity. The optical beam path is specifically designed for the purposes of confocal imaging leading to a short beam path length that fulfills the footprint requirements. The system allows an optical 3D scanning through the sample of up to 100 depths of focus without moving the sample. The scanning unit consists of a 2D MEMS scanning mirror spanning and a deformable mirror forming 3 virtual scanning axes. For a compact integration of the detection beam path, a confocal detector with an actuated MEMS pinhole was developed to adjust the optical sectioning. The selected light sources are directly modulated lasers operating at wavelengths that are frequently used for fluorescence imaging in life science applications. To provide a simple interface to almost any user’s hardware such as laptops or tablets, the systems architecture for real time control and data acquisition is based on a FPGA.

Citation Download Citation

Stefan Richter, Carsten Wehe, Uwe Wolf, Thomas Nobis, Lars-Christian Wittig, Philipp Huebner, Alexander Froeber, Gerhard Krampert, Dirk Thiele, Joerg Muetze, and Michael Kempe "Integrated confocal imaging system", Proc. SPIE 10931, MOEMS and Miniaturized Systems XVIII, 109310K (4 March 2019); https://doi.org/10.1117/12.2514552

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