Fluorescence spectroscopy approach for blood influence compensation

Valery Shupletsov; Viktor Dremin; Evgeny Zherebtsov; Mikhail Mezentsev; Igor Kozlov; Elena Potapova; Andrey Dunaev

doi:10.1117/12.2522794

3 June 2019 Fluorescence spectroscopy approach for blood influence compensation

Valery Shupletsov, Viktor Dremin, Evgeny Zherebtsov, Mikhail Mezentsev, Igor Kozlov, Elena Potapova, Andrey Dunaev

Proceedings Volume 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine; 110650R (2019) https://doi.org/10.1117/12.2522794
Event: International Symposium on Optics and Biophotonics VI: Saratov Fall Meeting 2018, 2018, Saratov, Russian Federation

Abstract

At present, fluorescence spectroscopy (FS) and diffuse reflectance spectroscopy (DRS) are widespread methods highly used in medical practice. The combined application of these methods is a promising tool to improve the predictive force of classifiers for tissue type recognition as well as to compensate the attenuation of the fluorescence radiation by blood for the accurate evaluation of the biomarkers content in living tissue. Several techniques are known to normalize the resulting fluorescence spectrum in order to exclude the attenuation effect. In this study, an approach based on the dividing of the experimentally obtained FS spectrum by DRS spectrum has been applied for experiments with occlusion test. The implemented multimodal approach for the in vivo optical measurements in combination with occlusion test for minimisation of blood influence has shown good repeatability of obtained experimental fluorescence spectra. The results are of particular interest for the further development of methods for compensating the influence of chromophores in optical spectroscopy.

Citation Download Citation

Valery Shupletsov, Viktor Dremin, Evgeny Zherebtsov, Mikhail Mezentsev, Igor Kozlov, Elena Potapova, and Andrey Dunaev "Fluorescence spectroscopy approach for blood influence compensation", Proc. SPIE 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, 110650R (3 June 2019); https://doi.org/10.1117/12.2522794

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