Generalized temporal sampling with active illumination in optical microscopy

Christian Jaques; Michael Liebling

doi:10.1117/12.2529526

9 September 2019 Generalized temporal sampling with active illumination in optical microscopy

Christian Jaques, Michael Liebling

Proceedings Volume 11138, Wavelets and Sparsity XVIII; 1113816 (2019) https://doi.org/10.1117/12.2529526
Event: SPIE Optical Engineering + Applications, 2019, San Diego, California, United States

Abstract

Generalized sampling is a flexible framework for signal acquisition, which relaxes the need for ideal pre-filters. Nevertheless, implementation remains challenging for dynamic imaging applications because it requires simultaneously measuring multiple overlapping inner-products and because only positive signals (intensities) can be measured by cameras. We present a method to collect videos of monochromatic objects by projecting the incoming signal at each pixel in a temporal B-spline space of degree 0, 1, or 2 by using a conventional RGB camera and a modulated three-color light source for illumination. Specifically, we solve the basis function overlap problem by multiplexing the acquisition in different color ranges and use B-spline pieces (which are positive) as projection kernels of a biorthogonal projection-expansion bases pair. The steps to recover signal samples include spectral unmixing and inverse filtering. Reconstructions we obtained from simulated and experimentally-acquired microscopy data demonstrate the feasibility of our approach.

Citation Download Citation

Christian Jaques and Michael Liebling "Generalized temporal sampling with active illumination in optical microscopy", Proc. SPIE 11138, Wavelets and Sparsity XVIII, 1113816 (9 September 2019); https://doi.org/10.1117/12.2529526

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