25 plane multifocus microscopy for fast and live 3D imaging (Conference Presentation)

Eduardo Hirata Miyasaki; Gustav M. Pettersson; Khant Zaw; Demis D. John; Brian Thibeault; Brandon Lynch; Juliana Hernandez; Sara Abrahamsson

doi:10.1117/12.2550979

9 March 2020 25 plane multifocus microscopy for fast and live 3D imaging (Conference Presentation)

Eduardo Hirata Miyasaki, Gustav M. Pettersson, Khant Zaw, Demis D. John, Brian Thibeault, Brandon Lynch, Juliana Hernandez, Sara Abrahamsson

Author Affiliations +

Proceedings Volume 11226, Neural Imaging and Sensing 2020; 1122602 (2020) https://doi.org/10.1117/12.2550979
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We here report an optical system for live 3D microscopy that we call the Multifocus 25-camera microscope (M25). M25 is a new design for aberration-corrected multifocus microscopy (MFM) that employs the latest generation of small, fast and sensitive CMOS cameras. Each of the 25 focal planes is captured on one of the individual cameras, enabling truly simultaneous 3D recording at >100Hz. M25 is built from a combination of custom manufactured diffractive Fourier optics elements and off-the-shelf components. We are employing M25 to study neural circuit function in small model organisms including C. elegans, Drosophila, and fish.

Conference Presentation

Citation Download Citation

Eduardo Hirata Miyasaki, Gustav M. Pettersson, Khant Zaw, Demis D. John, Brian Thibeault, Brandon Lynch, Juliana Hernandez, and Sara Abrahamsson "25 plane multifocus microscopy for fast and live 3D imaging (Conference Presentation)", Proc. SPIE 11226, Neural Imaging and Sensing 2020, 1122602 (9 March 2020); https://doi.org/10.1117/12.2550979

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