Digital histology of tissue with Mueller polarimetric microscopy (Conference Presentation)

Hee Ryung Lee; Pengcheng Li; Hui Ma; Christian Lotz; Florian Kai Groeber-Becker; Sofia Dembski; Razvigor Ossikovski; Tatiana Novikova

doi:10.1117/12.2543976

10 March 2020 Digital histology of tissue with Mueller polarimetric microscopy (Conference Presentation)

Hee Ryung Lee, Pengcheng Li, Hui Ma, Christian Lotz, Florian Kai Groeber-Becker, Sofia Dembski, Razvigor Ossikovski, Tatiana Novikova

Author Affiliations +

Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 1125111 (2020) https://doi.org/10.1117/12.2543976
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Tissue-based diagnostic relies on histological analysis of tissue cuts by a pathologist who links data derived from microscopy image content to a specific disease. We explored the potential of Mueller microscopy combined with density-based spatial clustering algorithm for applications with noise for the automated analysis of tissue histological cuts. Mueller microscopic images of unstained artificial human skin histological cuts were analyzed by applying differential Mueller matrix decomposition. Scalar retardance, depolarization and total transmitted intensity images were used for the automated segmentation of microscopic images. An algorithm of polarimetric data post-processing which mitigates the impact of tissue thickness fluctuations was suggested.

Conference Presentation

Citation Download Citation

Hee Ryung Lee, Pengcheng Li, Hui Ma, Christian Lotz, Florian Kai Groeber-Becker, Sofia Dembski, Razvigor Ossikovski, and Tatiana Novikova "Digital histology of tissue with Mueller polarimetric microscopy (Conference Presentation)", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 1125111 (10 March 2020); https://doi.org/10.1117/12.2543976

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available