Probing the mechanisms of infrared neural stimulation with stimulated Raman scattering microscopy (Conference Presentation)

Wilson R. Adams; Rekha Gautam; Graham A. Throckmorton; Laura E. Masson; Jeremy B. Ford; John Logan Jenkins; E. Duco Jansen; Anita Mahadevan-Jansen

doi:10.1117/12.2545228

10 March 2020 Probing the mechanisms of infrared neural stimulation with stimulated Raman scattering microscopy (Conference Presentation)

Wilson R. Adams, Rekha Gautam, Graham A. Throckmorton, Laura E. Masson, Jeremy B. Ford, John Logan Jenkins, E. Duco Jansen, Anita Mahadevan-Jansen

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Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 1125204 (2020) https://doi.org/10.1117/12.2545228
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Stimulated Raman scattering (SRS) offers a drastic speed advantage over conventional vibrational spectroscopic imaging techniques – making it ideal for studying fast biochemical dynamics. We developed an experimental paradigm that applies spectral stimulated Raman scattering (SRS) imaging to study the mechanisms of infrared (IR) photostimulation of neuronal cells. Infrared neural stimulation (INS) is a label-free optical neuromodulation technique with high spatial and temporal precision. Using SRS, changes in lipid and water vibrational signatures in live cells during INS were observed, suggesting that lipid membrane deformation accompanies IR exposure. The speeds afforded by SRS enables unprecedented observation of fast cellular biophysical dynamics.

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Wilson R. Adams, Rekha Gautam, Graham A. Throckmorton, Laura E. Masson, Jeremy B. Ford, John Logan Jenkins, E. Duco Jansen, and Anita Mahadevan-Jansen "Probing the mechanisms of infrared neural stimulation with stimulated Raman scattering microscopy (Conference Presentation)", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125204 (10 March 2020); https://doi.org/10.1117/12.2545228

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KEYWORDS

Infrared imaging

Infrared radiation

Raman scattering

Microscopy

Electrodynamics

Imaging spectroscopy

Infrared signatures

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