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10 March 2020 In vivo quantification of mean corpuscular hemoglobin concentration by transient absorption microscopy and stimulated Raman scattering microscopy (Conference Presentation)
Andrew Francis
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Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 1125208 (2020) https://doi.org/10.1117/12.2546665
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Hematocrit, the volume fraction of red blood cells in whole blood, is a crucial metric of animal health in pharmacokinetic and disease model experiments. While robust, current methods for measuring hematocrit can be prohibitory for longitudinal animal models where animal well-being restricts serial blood collection volumes. Here we present in vivo hemoglobin concentration quantification in a mouse ear model using bimodal ratiometric imaging of transient absorption of hemoglobin and stimulated Raman scattering of water. Additionally, we leverage the intrinsic high resolution of nonlinear imaging to demonstrate a low volume method for ex vivo size and volume quantification.
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Andrew Francis "In vivo quantification of mean corpuscular hemoglobin concentration by transient absorption microscopy and stimulated Raman scattering microscopy (Conference Presentation)", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125208 (10 March 2020); https://doi.org/10.1117/12.2546665
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KEYWORDS
Microscopy
Absorption
Blood
In vivo imaging
Raman scattering
Animal model studies
Ear
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