AIEgen-assisted STED nanoscopy and its application for super-resolved cellular visualization (Conference Presentation)

Dongyu Li; Dan Ding; Ben Zhong Tang; Junle Qu; Jun Qian; Dan Zhu

doi:10.1117/12.2566129

2 April 2020 AIEgen-assisted STED nanoscopy and its application for super-resolved cellular visualization (Conference Presentation)

Dongyu Li, Dan Ding, Ben Zhong Tang, Junle Qu, Jun Qian, Dan Zhu

Author Affiliations +

Proceedings Volume 11363, Tissue Optics and Photonics; 113632F (2020) https://doi.org/10.1117/12.2566129
Event: SPIE Photonics Europe, 2020, Online Only

Abstract

Stimulated emission depletion (STED) nanoscopy is a typical super-resolution imaging technique that has become a powerful tool for visualizing intracellular structures on the nanometer scale. Aggregation-induced emission luminogens (AIEgens) are ideal fluorescent agents for bioimaging and have been widely used for organelle targeting, cellular mapping and tracing. Since AIEgens generally have a large Stokes’ shift, which is beneficial for restraining the fluorescence background induced by the STED light, as well as high photobleaching resistance in their nanoaggregate states, which provides the potential for long-term imaging under a STED beam with high power density, they are ideal fluorescent agents for STED nanoscopy. The STED efficiency of aggregated TPA-T-CyP could reach more than 80%, and the dynamic mitochondrial visualization was achieved on the nanometer scale. Their moving, fission and fussion of mitochondria was clearly observed with a lateral spatial resolution of 74 nm.

Conference Presentation

Citation Download Citation

Dongyu Li, Dan Ding, Ben Zhong Tang, Junle Qu, Jun Qian, and Dan Zhu "AIEgen-assisted STED nanoscopy and its application for super-resolved cellular visualization (Conference Presentation)", Proc. SPIE 11363, Tissue Optics and Photonics, 113632F (2 April 2020); https://doi.org/10.1117/12.2566129

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available