Optimization of brain tissue section preparation and raman spectroscopy measurement protocols

Hülya Torun; Buse Bilgin; Kemal Baysal; İbrahim Kulaç; İhsan Solaroğlu; Mehmet Cengiz Onbaşlı

doi:10.1117/12.2567118

20 August 2020 Optimization of brain tissue section preparation and raman spectroscopy measurement protocols

Hülya Torun, Buse Bilgin, Kemal Baysal, İbrahim Kulaç, İhsan Solaroğlu, Mehmet Cengiz Onbaşlı

Proceedings Volume 11468, Enhanced Spectroscopies and Nanoimaging 2020; 1146819 (2020) https://doi.org/10.1117/12.2567118
Event: SPIE Nanoscience + Engineering, 2020, Online Only

Conference Poster

Abstract

Raman spectroscopy is a highly sensitive and specific technique for identifying tissue compositions. Raman-based characterization of normal and abnormal tissues is impeded due to the variability in routine tissue preparation techniques, fluorescent background, and molecular heterogeneity. Thus, sample preparation and Raman measurement conditions for tissue sections must be optimized. Here, we present an optimized Raman protocol and sample preparation method for brain tissue sections. This protocol allows the characterization of tissues and recognition of brain tumors by refining laser power, accumulation/exposure times, excitation wavelength, glass/CaF2 substrate, deparaffinization solvent, and the thickness of sections.

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Hülya Torun, Buse Bilgin, Kemal Baysal, İbrahim Kulaç, İhsan Solaroğlu, and Mehmet Cengiz Onbaşlı "Optimization of brain tissue section preparation and raman spectroscopy measurement protocols", Proc. SPIE 11468, Enhanced Spectroscopies and Nanoimaging 2020, 1146819 (20 August 2020); https://doi.org/10.1117/12.2567118

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