Decrease laser power of STED nanoscopy by FLIM

Luwei Wang; Wei Yan; Yue Chen; Jia Zhang; Jialin Wang; Yong Guo; Zhigang Yang; Junle Qu

doi:10.1117/12.2572971

10 October 2020 Decrease laser power of STED nanoscopy by FLIM

Luwei Wang, Wei Yan, Yue Chen, Jia Zhang, Jialin Wang, Yong Guo, Zhigang Yang, Junle Qu

Proceedings Volume 11553, Optics in Health Care and Biomedical Optics X; 1155310 (2020) https://doi.org/10.1117/12.2572971
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

We propose a spatiotemporal modulation method to achieve super-resolution imaging at a depletion power two orders of magnitude lower than traditional counterpart. By increasing the pulse interval between excitation and depletion lasers, the fluorescence lifetime data contain the spatiotemporal information of confocal and STED photons at the same time. Two kinds of information are bounded by depletion pulse in a period of the pulse trains, and their intensity difference represents the stimulated emission intensity by donut-shaped depletion laser. Finally, low-power STED imaging with high image quality is realized by subtracting the enhanced stimulated emission intensity from the confocal one.

Conference Presentation

Citation Download Citation

Luwei Wang, Wei Yan, Yue Chen, Jia Zhang, Jialin Wang, Yong Guo, Zhigang Yang, and Junle Qu "Decrease laser power of STED nanoscopy by FLIM", Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 1155310 (10 October 2020); https://doi.org/10.1117/12.2572971

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