Improved multi-slice Fourier ptychographic diffraction tomography based on high-numerical-aperture illuminations

Jiasong Sun; Chao Zuo; Qian Chen

doi:10.1117/12.2580308

7 October 2020 Improved multi-slice Fourier ptychographic diffraction tomography based on high-numerical-aperture illuminations

Jiasong Sun, Chao Zuo, Qian Chen

Author Affiliations +

Proceedings Volume 11571, Optics Frontier Online 2020: Optics Imaging and Display; 115710R (2020) https://doi.org/10.1117/12.2580308
Event: Optics Frontiers Online 2020: Optics Imaging and Display (OFO-1), 2020, Shanghai, China

Abstract

Recently, with the increasing demand for high-accuracy three-dimensional (3D) imaging and localizing of the internal subcellular structures in cells, quantitative refractive index tomography technique has been developed and widely applied in various fields, such as molecular biology, biochemistry and cell biology. Thanks to the noninterference, large field of view and high-resolution advantages of traditional Fourier ptychographic microscopy (FPM) technique, quantitative phase tomography (QPT) method based on multi-slice (MS) FPM has attracted considerable attention lately. However, since the reported MS model is simply established on the traditional two-dimensional FPM, it is difficult to characterize the 3D diffractive propagation properties under large oblique illumination angles accurately. In order to solve this problem, an improved oblique phase correction function for 3D MSFPM is proposed in this paper. The numerical phase error under the large oblique illumination angle is compensated in the iterative reconstruction algorithm, and then the reconstruction accuracy of the 3D QPT can be improved.

Citation Download Citation

Jiasong Sun, Chao Zuo, and Qian Chen "Improved multi-slice Fourier ptychographic diffraction tomography based on high-numerical-aperture illuminations", Proc. SPIE 11571, Optics Frontier Online 2020: Optics Imaging and Display, 115710R (7 October 2020); https://doi.org/10.1117/12.2580308

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