Laser-induced heating modulates microglial calcium signaling

John Logan Jenkins; Wilson R. Adams; Jeremy B. Ford; Sam Evans; Anita M. Mahadevan-Jansen; Mark R. Hutchinson; Duco Jansen

doi:10.1117/12.2588775

5 March 2021 Laser-induced heating modulates microglial calcium signaling

John Logan Jenkins, Wilson R. Adams, Jeremy B. Ford, Sam Evans, Anita M. Mahadevan-Jansen, Mark R. Hutchinson, Duco Jansen

Author Affiliations +

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 1162913 (2021) https://doi.org/10.1117/12.2588775
Event: SPIE BiOS, 2021, Online Only

Abstract

Infrared neural modulation is an optical technique to stimulate or inhibit neural activity with pulsed infrared light (IR). This study focuses on effects of IR and heat on microglial calcium signaling, a main indicator of physiological changes within the immune-like cell. Widefield fluorescence microscopy quantified the intracellular calcium fluxes, cell death, and early apoptosis fluorescent indicators in BV2 immortalized microglia cell culture after irradiation. We have found that unique cellular responses are highly associated with different temperature increases, and the irradiance thresholds for these responses do not drive cells to Caspase-3 apoptosis nor necrotic cellular death.

Conference Presentation

Citation Download Citation

John Logan Jenkins, Wilson R. Adams, Jeremy B. Ford, Sam Evans, Anita M. Mahadevan-Jansen, Mark R. Hutchinson, and Duco Jansen "Laser-induced heating modulates microglial calcium signaling", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 1162913 (5 March 2021); https://doi.org/10.1117/12.2588775

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Calcium

Cell death

Modulation

Infrared radiation

Forward looking infrared

Luminescence

Microscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years