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Hyperspectral imaging has emerged as a promising diagnostic technique in the medical field. However, reflection from a sample often consists of a combination of surface reflection (also known as glare) and volume reflection. In this study, we propose a method to separate these two by illuminating the samples from three different angles and using a least squares optimization. This widely applicable method showed an adequate distinction between surface and volume reflectance in optical phantoms as well as in breast tissue samples.
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Lynn-Jade Jong, Freija Geldof, Anouk Post, Henricus Sterenborg, Theo Ruers M.D., "Separating surface reflectance from volume reflectance in hyperspectral imaging," Proc. SPIE 11640, Optical Interactions with Tissue and Cells XXXII, 1164007 (5 March 2021); https://doi.org/10.1117/12.2578337