Skin cancer detection with a compact multimodal fiber laser multiphoton FLIM tomograph

Karsten König; Dmitry Pankin; Arun Paudel; Holger Hänßle; Julia K. Winkler; Michael Zieger; Martin Kaatz

doi:10.1117/12.2577608

5 March 2021 Skin cancer detection with a compact multimodal fiber laser multiphoton FLIM tomograph

Karsten König, Dmitry Pankin, Arun Paudel, Holger Hänßle, Julia K. Winkler, Michael Zieger, Martin Kaatz

Author Affiliations +

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480A (2021) https://doi.org/10.1117/12.2577608
Event: SPIE BiOS, 2021, Online Only

Abstract

A clinical multicenter study has been performed on patients with suspicious, potentially malignant, pigmented skin lesions to evaluate the femtosecond fiber laser based compact multiphoton tomograph MPTcompact for high-resolution clinical imaging. Label-free high-resolution optical skin biopsies have been obtained based on two-photon autofluorescence excited at 780 nm, second harmonic generation, dermoscopy with an additional white light imager, and confocal reflectance with the NIR ultrashort laser pulses at 80 MHz. Furthermore, fluorescence lifetime imaging (FLIM) based on time-correlated single photon counting has been performed. Here we report on first clinical results. Multimodal compact multiphoton 3D/4D tomographs may become important label-free clinical highresolution imaging tools to replace in part conventional skin histology based on physically taken tissue samples, fixation, slicing, staining, and 2D light microscopy.

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Citation Download Citation

Karsten König, Dmitry Pankin, Arun Paudel, Holger Hänßle, Julia K. Winkler, Michael Zieger, and Martin Kaatz "Skin cancer detection with a compact multimodal fiber laser multiphoton FLIM tomograph", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480A (5 March 2021); https://doi.org/10.1117/12.2577608

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