Image processing routine to analyze mitochondrial membrane potential in single cells with TMRE

Amani Gillette; Melissa Skala

doi:10.1117/12.2578847

5 March 2021 Image processing routine to analyze mitochondrial membrane potential in single cells with TMRE

Amani Gillette, Melissa Skala

Author Affiliations +

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116481D (2021) https://doi.org/10.1117/12.2578847
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

Mitochondrial outer membrane permeabilization (MOMP) is a decisive step in the execution of cell death, and MOMP is often altered in cancer cells. MOMP could provide an early measurement of cancer treatment efficacy, and a method to understand relationships between mitochondrial function and cell death. Cationic dyes such as TMRE label mitochondria in a membrane potential dependent manner and provide insight into mitochondrial function in living cells. Currently, there are no easy to use open-source analysis methods to rapidly quantify bulk mitochondrial membrane potentials on a per cell level. Here, we present a custom CellProfiler pipeline to isolate TMRE intensity in both the mitochondria and nuclei of cells for rapid cell level quantification of bulk membrane potential.

Conference Presentation

Citation Download Citation

Amani Gillette and Melissa Skala "Image processing routine to analyze mitochondrial membrane potential in single cells with TMRE", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116481D (5 March 2021); https://doi.org/10.1117/12.2578847

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