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Fluorescence-encoded Raman spectroscopy has become increasingly more popular by virtue of its high chemical specificity and sensitivity. However, current fluorescence-encoding methods are narrowband and lack sensitivity in the low wavenumber region which if addressed could further enhance these methods. To overcome these limitations, we propose and experimentally demonstrate a novel broadband method for fluorescence-encoded Raman spectroscopy, termed fluorescence-encoded time-domain coherent Raman spectroscopy (FLETCHERS), which is capable of probing molecular vibrations in the lower fingerprint region (200 – 750 cm-1 ) with sample concentrations as dilute as 100 nM and laser powers as low as 20 mW.
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Phillip Charles McCann, Kotaro Hiramatsu, Keisuke Goda, "Fluorescence-encoded time-domain coherent Raman spectroscopy through single-photon counting," Proc. SPIE 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV, 116500B (5 March 2021); https://doi.org/10.1117/12.2576638