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5 March 2021 Protein sensing in solution using a high-speed lens-free holographic microscope
Zhen Xiong, Euan McLeod
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Proceedings Volume 11651, Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics; 116510Q (2021) https://doi.org/10.1117/12.2578958
Event: SPIE BiOS, 2021, Online Only
Abstract
Lens-free holographic microscopy (LFHM) is cost-effective and field-portable, making it a promising diagnostic approach for point-of-care applications. However, LFHM has not yet been applied to protein molecule sensing in solution . Here we develop a quantitative large-area binding sensor by combining a high-speed LFHM with a one-step bead-based agglutination assay, where agglutination of >10^4 2-μm beads in solution undergoing Brownian motion are imaged and quantified. We sense NeutrAvidin molecules and interferon-gamma (an immune system biomarker) in solution, achieving a limit of detection of <27 ng/mL for NeutrAvidin and <3 ng/mL for mouse interferon-gamma.
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Zhen Xiong and Euan McLeod "Protein sensing in solution using a high-speed lens-free holographic microscope", Proc. SPIE 11651, Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics, 116510Q (5 March 2021); https://doi.org/10.1117/12.2578958
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KEYWORDS
Proteins
Holography
Microscopes
Sensors
Diagnostics
Image processing
Molecules
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