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Label-free intensity and lifetime two photon excited fluorescence in combination with second harmonic generation images yield a wealth of morphological and functional information. Quantitative assessments of such images enable characterization of cellular metabolism and morphology in the context of important structural features of the surrounding matrix and/or biomaterial scaffold. Dynamic changes and interactions that are difficult to characterize using traditional staining or bulk tissue assessment techniques are revealed in the context of disease presence, tissue responses, and cell-matrix interactions in human and engineered tissues.
Irene Georgakoudi
"Label free two photon imaging to assess tissue morphology and function", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550I (5 March 2021); https://doi.org/10.1117/12.2579875
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Irene Georgakoudi, "Label free two photon imaging to assess tissue morphology and function," Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550I (5 March 2021); https://doi.org/10.1117/12.2579875