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Infrared spectroscopic imaging is an analytical approach that can reveal important molecular information without the need for substantial sample processing. These instruments can provide objective and automated evaluations to aid pathologists improve diagnostic accuracy. The quantum cascade laser, allows for a discrete frequency approach, increasing imaging speeds with superior spatial and spectral resolution. We present our recent progress toward developing new instruments capable of diffraction limited performance at all fingerprint-region wavelengths across the entire field of view. We demonstrate high throughput imaging of tissue sections and tissue microarrays and evaluate the advantages in data quality obtained from a well-corrected system.
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Kevin L. Yeh, Yamuna Phal, Rohit Bhargava, "Infrared chemical imaging with scanning quantum cascade laser microscopy systems," Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116561A (8 March 2021); https://doi.org/10.1117/12.2578981